Profile
Our lab works in the area of computational systems and synthetic biology domain wherein we try to integrate the action of regulatory circuits, cross talk between different signaling/metabolic pathways and the non-linear kinetics of biochemical processes through mathematical modeling. The hypotheses laid down is further validated in experimental conditions (both in vitro and in vivo)
Current Focus Areas
Investigating the molecular processes that underpin cellular function and contribute to human disease is the focus of lab pertaining to systems and synthetic biology. To dissect these intricate biological findings into novel medical treatments, we integrate multidisciplinary approaches. The lab deals with Leishmaniasis (infection model) and Lung Cancer (inflammation model)
Selected Publications
1 Khandibharad S, Singh S*. (2024) Single-cell ATAC sequencing identifies sleepy macrophages during reciprocity of cytokines in L. major infection. Microbiol Spectr. 2024 Feb 1:e0347823. doi: 10.1128/spectrum.03478-23. Online ahead of print. PMID: 38299832
2. Guhe V, Singh S*. (2024) Targeting peptide based therapeutics: Integrated computational and experimental studies of autophagic regulation in host-parasite interaction. ChemMedChem. 2024 Feb 5:e202300679. doi: 10.1002/cmdc.202300679. Online ahead of print. PMID: 38317307
3. Jawale D, Khandibharad S, Singh S*. (2023) Decoding systems immunological model of sphingolipids with IL-6/IL-17/IL-23 axes in L. major infection. Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Feb;1868(2):159261. doi: 10.1016/j.bbalip.2022.159261. Epub 2022 Dec 7.PMID: 36494028
4. Khandibharad S, Singh S*. (2022) Artificial intelligence channelizing protein-peptide interactions pipeline for host-parasite paradigm in IL-10 and IL-12 reciprocity by SHP-1. Biochim Biophys Acta Mol Basis Dis. 2022 1868(10):166466. doi: 10.1016/j.bbadis.2022.166466. Epub 2022 Jun 21. PMID: 35750267
5. Gulhane P, Singh S*. (2022) MicroRNA-520c-3p impacts sphingolipid metabolism mediating PI3K/AKT signaling in NSCLC: Systems perspective. J Cell Biochem. 2022 Aug 17. doi: 10.1002/jcb.30319. Online ahead of print. PMID: 35977046
6.Kabra R, Ingale P, Singh Shailza*. Computationally designed synthetic peptides for transporter proteins impart allostericity in Miltefosine resistant L. major. Biochem J. 2020 May 29; 477(10):2007-2026. Doi: 10.1042/BCJ20200176. PMID: 32391551.
7. Mandlik V, Shinde S, Singh Shailza*. (2014) Molecular evolution of the enzymes involved in the sphingolipid metabolism of Leishmania: selection pressure in relation to functional divergence and conservation. BMC Evol Biol. 2014 Jun 21; 14(1):142. [Epub ahead of print] PMID: 24951280 [PubMed - as supplied by publisher]
8. Anurag Kumar, Prajakta Nimsarkar and Shailza Singh (2022) Probing the interactions responsible for the structural stability of Trypanothione reductase through Computer Simulation and Biophysical Characterization. Protein J. PMID: 35364760
9. Kumar A, Nimsarkar P, Singh S*. (2022) Systems pharmacology aiding benzimidazole scaffold as potential lead compounds against leishmaniasis for functional therapeutics. Life Sci. 2022 Sep 15:120960. doi: 10.1016/j.lfs.2022.120960. Online ahead of print. PMID: 36116527
10. Khilwani R, Singh S*. (2024) Traversing through the Mechanistic Event Analysis in IL-6 and IL-17 Signaling for a New Therapeutic Paradigm in NSCLC. Int J Mol Sci. 2024 Jan 19;25(2):1216. doi: 10.3390/ijms25021216.PMID: 38279220