Profile
My group is interested in understanding the role of clathrin mediated endocytosis in the context of development and disease. We have observed that for the maintenance of stem cell pluripotency, clathrin mediated endocytosis is required. Further, we observe that neurodegenerative disorders such as Huntington's disorder display stalled clathrin mediated endocytosis. Further mutations in the clathrin heavy chain result in neurodevelopmental disorders such as CLTC disorder. We use a combination of cellular and organismal models to address the role and function of clathrin in normal development and in disease.
Current Focus Areas
Understanding the role of clathrin mediated endocytosis in health and disease
Selected Publications
Surya Bansi Singh, Shatruhan Singh Rajput, Shivprasad Patil and Deepa Subramanyam*. Protocol for measuring mechanical properties of live cells using atomic force microscopy. STAR Protocols, 2024 Mar 15; 5(1):102870.
Surya Bansi Singh, Shatruhan Singh Rajput, Aditya Sharma, Vaishnavi Ananthanarayanan, Amitabha Nandi, Shivprasad Patil, Amitabha Majumdar and Deepa Subramanyam*. Pathogenic aggregates alter actin organization and cellular viscosity resulting in stalled clathrin mediated endocytosis. BioRxiv, 2023; 2023.07.10.548473; DOI: https://doi.org/10.1101/2023.07.10.548473.
Deepika Puri, Aparna Kelkar, Bhaskar Gauri Shankar and Deepa Subramanyam. Autophagy-cell death balance is maintained by Polycomb-mediated regulation during stem cell differentiation. FEBS J.; 2023, 290(6):1625-1644.
Ridim D Mote, Jyoti Yadav, Surya Bansi Singh, Mahak Tiwari, Laxmikant Shinde V, Shivprasad Patil and Deepa Subramanyam*. Pluripotency of embryonic stem cells lacking clathrin mediated endocytosis cannot be rescued by restoring cellular stiffness. J. Biol. Chem. 2020; 295(49): 16888-16896.
Yadavalli V Narayana, Chetan Gadgil, Ridim D. Mote, Raghav Rajan and Deepa Subramanyam*. Clathrin-mediated endocytosis regulates a balance between opposing signals to maintain the pluripotent state of embryonic stem cells. Stem Cell Reports. 2019; 12(1): 152-164.