Profile
RNA-Protein interactions are important in regulating gene expression. Several RNA binding proteins associate with insulin mRNA and regulate its expression. Protein disulfide isomerase, PolyA binding protein and HuD interacts with 5ÚTR of insulin mRNA and regulate glucose stimulated insulin biosynthesis. These factors also associate PC1/3 and PC2 mRNA to regulate the expression of pPc1 and PC2 pro convertases that are required for processing of insulin to generate a functional insulin molecule. Altered expression of these regulatory molecules may lead to dysregulated insulin expression leading to insulin resistance. We also have identified Phosphatidyl inositol 5-phosphate 4-kinase (PIP4K) as an RNA binding protein. PIP4K was shown to be imported into malaria parasite, where it associates with specific transcripts. Multiple UUGU motifs present in the target RNA are essential for interaction with PIP4K. The RNA binding activity of the PIP4K is conserved across species and it associates with several different RNA.
Current Focus Areas
Selected Publications
Rucha D. Sarwade, Abdul Khalique, Shardul D. Kulkarni, Poonam R. Pandey, Naina Gaikwad and Vasudevan Seshadri (2020) Translation of insulin granule proteins are regulated by PDI and PABP Biochem Biophys Res Commun. 526 (3), 618-625, 4 June 2020 https://doi.org/10.1016/j.bbrc.2020.03.106
Dandewad V, Vindu A, Joseph J and Seshadri V. (2019) Import of human miRNA-RISC complex into Plasmodium falciparum and regulation of the parasite gene expressio. Journal of Bioscience 44:50, DOI: 10.1007/s12038-019-9870-x
Vindu A, Dandewad V, Seshadri V. (2018) Identification of human Phosphatidyl Inositol 5-Phosphate 4-Kinase as an RNA binding protein that is imported into Plasmodium falciparum. Biochem Biophys Res Commun.;498(3):529-536. doi: 10.1016/j.bbrc.2018.03.014. Epub 2018 Mar 6.
Shehnaz Bano, Shyam More, Dattatray S. Mongad, Abdul Khalique, Dhiraj P. Dhotre, Manoj K. Bhat, Vasudevan Seshadri (2022) Prolonged exposure to insulin causes epigenetic alteration leading to insulin resistance (BioRxiv) doi: https://doi.org/10.1101/2022.04.28.489884
Pandey PR, Sarwade RD, Khalique A, Seshadri V. (2018) Interaction of HuDA and PABP at 5'UTR of mouse insulin2 regulates insulin biosynthesis. PLoS One;13(3):e0194482. doi: 10.1371/journal.pone.0194482. ECollection 2018
Jatin Behari, Pranita Borkar, Arya Vindu, Vishal Dandewad, Sindhuri Upadrasta, Dhanshekaran Shanmugam, Vasudevan Seshadri (2021) Conserved RNA binding activity of Phoshatidyl inositol 5-phosphate 4-kinase (PIP4K2) Frontiers in Molecular Biosciences doi.org/10.3389/fmolb.2021.631281