Profile
My lab is currently pursuing structural studies on human nuclear pore complexes using the intergrative approaches of structural biology, cryo-EM, biochemical reconstitution of the complexes and fluorescence microscopy. Currently we are pursuing following research projects:
Current Focus Areas
1. Biochemical reconstitution and cryo-EM studies on Nup93 subcomplex of the human NPC: Using both E coli and mammalian cells as expression host, we purify multi-Nup complexes and pursuuing theri 3D structure determination using cryo-EM methods
2. Elucidating the role of Nucleoporins in viral infections: human NPC play an important role in viral infection. Currently we are pursuing studies to understand molecular basis for viral entry into the nucleus
3. Developing computational tools for deciphering protein-protein interactions solely based on primary sequence:s Swe have developed a pipleline to map precide interscting domains of two partner proteins. We are further developing it to decipher further interactions between viral and host proteins
4. Developing tools for recombinant protein production for therapeutic purposes: As the lab has expertise in recombinant protein production using mammalian expression system, we aim to develop advances tools for large scale production of the therapeutics proteins
Selected Publications
Madheshiya P.K., Shukla E., Singh J., Bawaria S, Ansari M.Y., and Chauhan R. Insights into the role of Nup62 and Nup93 in assembling the cytoplasmic ring and central transport channel of the nuclear pore complex. Molecular Biology of the Cell 2022 33:14.
P. Sonawane*, P. Dewangan*, S. Neeranjan, P. Madheshiya, K. Chopra, S. Bawaria, M. Kumar, M. Banerjee and R. Chauhan 2020. Structural basis for Nup93 mediated anchoring of central transport channel of the nuclear pore complex. Protein Science 29(12) 2510-2527
K. Chopra, B Budrak, K. Sharma, A. Kembhavi, S.C. Mande and R. Chauhan 2020. A pipeline to decrypt the protein-protein interaction from amino acid sequence information. Biomolecules 10(6). 938-951
E. Shulka and R. Chauhan. 2019. Host-HIV-1 interactome: A quest for novel therapeutic interventions. Cells 8, 1155-1163.
K. Chopra, S. Bawaria and R. Chauhan (2019). Evolutionary divergence of the nuclear pore complex from fungi to metazoans. Protein Science 28(3); 571-586/. https://doi.org/10.1002/pro.3558