Profile
My research expertise is in the synthesis of small molecular fluorescent chemical probes, designing DNA-based fluorescence reporters and electrochemical DNA biosensors for cell imaging and biomarkers detection applications in living systems and biological fluids (blood, saliva, sweat, urine and cerebrospinal fluid). Our lab at RGCB primarily focuses on developing new activity-based fluorescence probes/bioconjugation tools to uncover the new biology of non-enzymatic protein-posttranslational modifications (PTMs), non-apoptotic cell death forms and intracellular organelle membrane contact sites (MCS). Further, we are also interested in developing DNA-based biosensors for detecting small molecular and protein biomarkers in liquid biopsy samples.
Current Focus Areas
Our lab primarily focuses on developing new bioconjugation and fluorescence tools to understand the role of non-enzymatic protein-posttranslational modifications in the context of diabetes and metabolic disorders.
We are also interested in applying cutting-edge fluorescence imaging tools to study non-apoptotic cell death pathways and identify the potential biomarkers and drug targets for drug-resistance cancer’s, cardiovascular and neurodegenerative diseases.
DNA-based biosensors have achieved rapid, sensitive, quantitative and multiplexed detection of biological and pharmacological cues in serum and whole blood. Our lab is also interested in developing DNA-based pattern ID probes to detect small molecule and protein biomarkers for cardiovascular and neurodegenerative diseases.
Selected Publications
Narayanaswamy, N., Chakraborty, K., Saminathan, A., Zeichner, E., Leung, KH., Devany, J., Krishnan, Y. (2019). A pH-correctable, DNA-based fluorescent reporter for organellar calcium. Nature Methods, 16, 95-102.
Rajasekhar, K., Narayanaswamy, N., Murugan, N.A., Viccaro, K., Lee, H-G., Shah, K., Govindaraju, T. (2017). Aβ plaque-selective NIR fluorescence probe to differentiate Alzheimer's disease from tauopathies. Biosensors and Bioelectronics, 98, 54-61.
Narayanaswamy, N., Narra, S. Nair, R.R., Saini, D.K., Kondaiah, P., Govindaraju, T. (2016). Stimuli-responsive colorimetric and NIR fluorescence combination probe for selective reporting of cellular hydrogen peroxide. Chemical Science, 7, 2832-2841.
Narayanaswamy, N., Das, S., Samanta, P.K., Banu, K., Sharma, G.P., Mondal, N., Dhar, S.K., Pati, S.K., Govindaraju, T. (2015). Sequence-specific recognition of DNA minor groove by an NIR-fluorescence switch-on probe and its potential applications. Nucleic acids research, 43, 8651-8663.
Narayanaswamy, N., Kumar, M., Das, S., Sharma, R., Samanta, P.K., Pati, S.K., Dhar, S.K., Tapas K Kundu, T.K., Govindaraju, T. (2014). A thiazole coumarin (TC) turn-on fluorescence probe for AT-base pair detection and multipurpose applications in different biological systems. Scientific Reports, 4, 6476.