Profile
Dr. Nirmalya Ganguli did his Bachelor’s and Master’s Degree from Bangalore University. He obtained his Ph.D. degree from the University of Delhi South Campus, and his doctoral work was focused on Direct Delivery of Transgene in the Mammary Gland for production of Therapeutic Protein. He worked as Research Associate at the National Institute of Immunology, New Delhi, on developing easy methods of transgenesis through testicular route as well as Spermatogonial Stem Cells/Germ Cell transplantation study. He joined NIAB in the year 2017. Dr. Ganguli is working on development of genetically engineered livestock for agricultural applications and biomedical research. He has specialization in the area of Transgenesis, Generation of Animal Bioreactor, Direct Mammary Gland Transfection, Genome and Protein Engineering. He is experienced in the generation of transgenic mice, generation of gene knockdown mice using shRNA, genome engineering through CRISPR-Cas system, generation of various ubiquitous/tissue specific mammalian expression vector, production of therapeutic protein in milk, and testicular germ cell depletion & stem cell transplantation work.
Current Focus Areas
1. Adapting the Testicular Transgenesis in Farm Animals.
2. Generation of Farm Animal Bioreactor for Production of Biotherapeutics.
3. Development of Cell Line for enhancing Bio Manufacturing.
4. Generation of Genome Engineered Animal for increasing Productivity and functional Genomics Studies.
Selected Publications
Goat mammary epithelial cells provide a better expression system for production of recombinant human bone morphogenetic protein 2 compared to Chinese hamster ovarian cells. Venkateswaran Ganesan, Goutam Ulgekar, Anandhi Ramalingam, Souvik Sen Sharma, Nirmalya Ganguli*, Subeer S. Majumdar*. Cell Biochem Funct. 2024;42:e3982. doi:10.1002/cbf.3982. (*Co-Correspondence Author)
Isolation and characterisation of promoters from mouse genome to drive post-meiotic germ cell-specific robust gene expression for functional genomics studies. Abhishek Das, Srimoyee Koner, Subeer S. Majumdar, Nirmalya Ganguli. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. Volume 1867, Issue 1, 2024, 194994, ISSN 1874-9399, https://doi.org/10.1016/j.bbagrm.2023.194994.
A Combinatorial Approach for Robust Transgene Delivery and Targeted Expression in Mammary Gland for Generating Biotherapeutics in milk, Bypassing Germline Gene Integration. Nirmalya Ganguli, Nilanjana Ganguli, Sunandini Chandra, Mayank Chaubey, Debi P. Sarkar and Subeer S. Majumdar. Jouranl of Applied Microbiology and Biotechnology. 2018 Jul;102(14):6221-6234. doi: 10.1007/s00253-018-9094-2.
An efficient method for generating a germ cell depleted animal model for studies related to spermatogonial stem cell transplantation. Nirmalya Ganguli, Neerja Wadhwa, Abul Usmani, Neetu Kunj, Nilanjana Ganguli, Rajesh Kumar Sarkar, Soma M Ghorai, Subeer S Majumdar. Stem Cell Research and Therapy, 2016, 7: 142. doi:10.1186/s13287-016-0405-1
A non-surgical approach for male germ cell mediated gene transmission through transgenesis. Abul Usmani, Nirmalya Ganguli, Hironmoy Sarkar, Suveera Dhup, Suryaprakash R. Batta, Manoj Vimal, Nilanjana Ganguli, Sayon Basu, P. Nagarajan and Subeer S. Majumdar. Scientific Reports, 2013, 3, Article number: 3430 doi: 10.1038/srep03430.